Agarose 3%, 250ml, 0.5xTBE (Fresh soln for tank)
Starting Voltage: ~40 mA switched to 30 mA after 22 min, switched to 40 mA after ~2 hrs 40 min, switched to 50 mA after 1 hr.
Pour @ 8:44am
Load samples 9:20 am
Run time: 5 hrs (10:00 – 3:00)
A1-12, B1-5, G4, B7-12, Ladder, C1-2, G1,G3,C12 – top row
D1-12. E1-5, G6, E7-12, F1,F3,G5,G7,F12 – middle row
Results: Faint bands visible for 118/119 after 25 min staining, ladder also faint, left for additional 5min. Bands were only visible for samples with Biolase, not samples with only the French polymerase. No bands for 27/28 with either polymerase.
