3% agarose gel (7.5g) poured Dec4th, 250ml 0.5x TBE
Starting current: 46 mA
Load samples @ 8:55am
Top Row: A1-12, ladder, B1-12, C1-2, F1-2,G1 (PCRdec0407-1p)
Middle Row: C12, F3-4,G3 (same pcr), Ladder, G1-8, B7-12, F11 (PCRnov3007-2p)
Run start : 9:12am
10:40(checked current @ 46mA), 11:39 (changed current from 47 to 54mA)
Run end: 12:55pm
Staining: 12:57pm-1:18pm, checked again @ 1:49pm- no improvement
Results: Bands visible only for B5-7, F3-4, G3, and very faint for samples from PCRnov3007-2p (G3,4 B7,8,12); ladder is still faint/somewhat streaky.
(F3-4, G3=JA 1/21 FPsample)
