Chemicals used (approximation) per 100 extracts
(for modified Kobayashi et al. protocol)
70 ml CIAA
100 ml Isopropanol
100ml Ethanol
30 ml Sorbitol (1.75 M)
0.15 ml 2-Mercapto-ethanol
10 g PEG 6000
1.5 ml 0.5M EDTA pH8
15 ml 0.5M Tris-HCl pH 8
0.15 g PVPP
5 ml 10% N-Lauroyl sarcosine
7.1 ml 5M NaCl
2.5ml 1% CTAB
Notes
D-squared note: Overnight pptn of DNA ok. Pellets unaffected.
EDTA Used in March 2002: Disodium dihydrate
Gel quantitation: Dryloading works well only if yield is good.
